HEMOCOMPATIBILITY STUDIES OF LAYER-BY-LAYER POLYELECTROLYTE COMPLEXES FOR BIO-BASED POLYMERS

Mthabisi Talent George Moyo; Terin Adali; Oğuz Han Edebal; Ece Bayir; Aylin Şendemir

doi:10.17222/mit.2023.922

Mthabisi Talent George Moyo Department of Biomedical Engineering, Faculty of Engineering, Near East University, P.O. Box: 99138, TRNC, Mersin 10 Turkey
Terin Adali Department of Medical Biochemistry, Faculty of Medicine, Girne American University, P.O. Box: 99320 Karaman Girne, Turkish Republic of Northern Cyprus (TRNC), Mersin 10 Turkey.
Oğuz Han Edebal Clinical Biochemistry Laboratory, Near East University Hospital, P.O. Box: 99138, Turkish Republic of Northern Cyprus (TRNC), Mersin 10 Turkey
Ece Bayir Department of Bioengineering, Faculty of Engineering, Ege University, Bornova, 35100 Izmir, Turkey
Aylin Şendemir Department of Bioengineering, Faculty of Engineering, Ege University, Bornova, 35100 Izmir, Turkey

DOI: https://doi.org/10.17222/mit.2023.922

Keywords: polyelectrolyte complex, hemocompatibility, thrombogenesis

Abstract

Thrombogenesis is an important issue that causes blood-contacting biomedical device failure. This study focuses on hemocompatibility studies of novel blood-contacting polyelectrolyte complexes (PECs) for biomedical application designs. PEC films were fabricated from biobased polymers of silk fibroin (SF), chitosan (CH), and sodium alginate (AL) through the solvent casting method as well as Layer-by-Layer (LbL) technique. Characterization was carried out by Fourier-transform infrared spectroscopy (FTIR), Scanning electron microscopy (SEM), Atomic force microscopy (AFM), and Differential scanning calorimetry (DSC) analyses. FTIR spectra displayed all layers’ characteristic peaks of SF, CH, and AL. AFM images indicated that the addition of AL as an outer layer increased surface roughness. DSC analysis suggested that the best thermal stability has been observed with the CH outer layer of PECs. SEM micrograph analysis indicated that the morphologies of PECs were affected by the inclusion of the clopidogrel bisulfate (CLB). Hemocompatibility properties were investigated by complete blood count (CBC), prothrombin time (PT), international normalized ratio (INR), activated partial thromboplastin time (APTT), platelet adhesion, erythrocyte morphology analysis, in vitro cholesterol, and albumin level tests. These hemocompatibility analyses demonstrated that the PEC surfaces provide favourable principles to design and develop non-thrombogenic PECs for blood-contacting biomedical applications

Author Biographies

Terin Adali, Department of Medical Biochemistry, Faculty of Medicine, Girne American University, P.O. Box: 99320 Karaman Girne, Turkish Republic of Northern Cyprus (TRNC), Mersin 10 Turkey.

Department of Biomedical Engineering, Faculty of Engineering, Near East University, P.O. Box: 99138, TRNC, Mersin 10 Turkey

Oğuz Han Edebal, Clinical Biochemistry Laboratory, Near East University Hospital, P.O. Box: 99138, Turkish Republic of Northern Cyprus (TRNC), Mersin 10 Turkey

Medical doctor in Biochemistry. Near East University Hospital, Clinical Biochemistry Laboratory, TRNC, Mersin 10 Turkey

Ece Bayir, Department of Bioengineering, Faculty of Engineering, Ege University, Bornova, 35100 Izmir, Turkey

Researcher at Central Research Test and Analysis Laboratory Application and Research Center (EGE-MATAL), Ege University, Bornova, 35100 Izmir, Turkey

Aylin Şendemir, Department of Bioengineering, Faculty of Engineering, Ege University, Bornova, 35100 Izmir, Turkey

Head of Department of Bioengineering, Faculty of Engineering, Ege University, Bornova, 35100 Izmir, Turkey

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